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. 2020 Jun 17;13:5709–5719. doi: 10.2147/OTT.S231076

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© 2020 Guo et al.

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(A) Hsa_circ_0036722 was downregulated in AMC-HN-8 cell line transfected with siRNA targeting its junction sequence (***P < 0.0001). (B and C) MiR-1248 was upregulated (***P < 0.0001) while RHCG (*P < 0.05) was downregulated after hsa_circ_0036722 was downregulated in AMC-HN-8 cell line. (D) The expression of miR-1248 was decreased by miR-1248 inhibitors, and the transfection efficiency was examined by qRT-PCR (*P < 0.05). (E) Downregulation of miR-1248 significantly increased the expression of RHCG (*P < 0.05). (F) Co-transfection of miR-1248 inhibitors could partly reverse the RHCG decrease induced by downregulated hsa_circ_0036722 (**P < 0.01). Higher values of 2^−ΔΔCt represented relatively higher expression levels in this figure. (G and H) Luciferase reporter assay showed that ectopic expression of miR-1248 could suppress the activity of hsa_circ_0036722-WT (***P < 0.001) and RHCG-WT (**P < 0.01) reporters, respectively.