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. 2020 Jun 17;15:4275–4288. doi: 10.2147/IJN.S255833

Figure 3.

Figure 3

(A) colony-forming units (CFU) assay using E. coli after treatment with eADF4(κ16) and PVA-coated Se NPs with varying concentrations from 3.9 µg/mL to 250 µg/mL. One-way ANOVA with Tukey’s post hoc test was used to compare means of experimental groups at each concentration, *p-value < 0.05. (B–D) Agar plate images of CFU test of E. coli, (B) control without particles, (C) PVA coated Se NPs, (D) eADF4(κ16)-coated Se NPs. 10° is the original (bacteria + Se NPs) solution, 10−1, 10−2, 10−3 and 10−4 mean diluting the original solution 10, 100, 1000 and 10,000 times, respectively, to make the colonies more countable. SEM images of 2.5 ×107 cells/mL E. coli before and after treatment with 75 µg/mL Se NPs: (E) plain E. coli, (F) E. coli incubated with PVA coated Se NPs, and (G) E. coli incubated with eADF4(κ16)-coated Se NPs.