Figure 5.

FKA induced ROS generation in primary splenocytes. (a) Splenocytes were pretreated with FKA (30 μM) for 0-60 min. The generation of intracellular ROS levels was indicated by the DCF fluorescence method that was measured by flow cytometry. (b) Data were presented as fold change of ROS over the FKA untreated control cells. Results were represented as the mean ± SD of three experiments. Statistical significance was assigned as ^∗p < 0.05, ^∗∗p < 0.01, and ^∗∗∗p < 0.001 compared to the control cells.