Skip to main content
. Author manuscript; available in PMC: 2021 Jun 15.
Published in final edited form as: J Immunol. 2020 Apr 24;204(12):3117–3128. doi: 10.4049/jimmunol.1900391

FIGURE 5.

FIGURE 5.

Blockade of BAFF alters BCR repertoire during hepatic fibrosis. Anti-BAFF mAb or sterile PBS were administered into Mdr2−/− mice once a week starting at 8 weeks until 25 weeks of age. Total B lymphocytes (CD19+MHCII+CD5-) were enriched by FACS sorting and analyzed for BCR IgH repertoire. Comparison of BCR repertoire from anti-BAFF (n=3) and PBS (n=3) treated Mdr2−/− animals were performed using (A) productive templates analysis, (B) Simpson’s clonality estimation, and (C) Daley Smith Estimator. (D) The stacked bar graph showing the frequency of top ten clonotypes found in anti-BAFF and PBS treated Mdr2−/− animals. (E) Morisita analysis showing heatmap of overlap among top ten clonotypes found in anti-BAFF and PBS treated Mdr2−/− animals. Statistical analysis was performed by unpaired Student t test for panel A. *P<.05, **P<.001. Error bars reflect SEM.