Fig.1.

Generation of the APP-P2A-GFP lentiviral vector. A) Design of the APP mutations. The Swedish, Arctic and Iberian mutations were introduced into the human APP gene. B) Schematic representation of the lentiviral vector used for generation of APP Tg monkeys. LTR, long terminal repeat; S, signal peptide sequence; FLAG, FLAG epitope tag sequence; P2A, self-cleaving 2A peptide sequence; GFP, green fluorescent protein; WPRE, woodchuck hepatitis posttranscriptional regulatory element. C) Localization of FLAG-tagged APP protein. Scale bars = 10 μm. D) Fluorescent images of 293FT cells 48 h after transduction with an APP-P2A-GFP lentivirus. Insets in each panel show brightfield images. Scale bars = 100 μm. E) APP protein expression in the lentivirus-infected 293FT cells and culture supernatants. APP (C), APP-C terminal.