Fig.2.

Generation of APP-GFP Tg cynomolgus monkeys. A) Images of cynomolgus monkey blastocysts 7 days after infection with the lentivirus. Scale bars = 100 μm. The left panel shows a brightfield image and the right panel shows a fluorescence image. B) Immunohistochemistry of the blastocysts 7 days after infection with anti-CDX2 and GFP antibodies. Scale bars = 100 μm. C) Images of vitrified and thawed cynomolgus monkey blastocysts. Upper panels show expanded blastocysts at 12 h after thawing. Lower panels show unexpanded blastocysts at the same time. D) Efficiency of recovery rate after vitrification and thawing. E) Implantation rate of WT blastocysts, APP lentivirus-infected blastocyst (one or two embryos per recipient). **p < 0.01 between treatments.