Fig.3.

GFP expression of aborted twin APP Tg monkeys. A) Bright-field and fluorescence microscopy images of the fetal Tg monkeys. B) Detection of the integrated transgene by genomic PCR. DNA from a normal placenta was used as a control. C) Detection of the transcript from the transgene by RT–PCR. RNA from a normal placenta was used as a control. D) Immunohistochemistry of the cerebral cortex of the aborted Tg and age-matched non-transgenic fetuses. Sections of the cerebral cortex were subjected to immunohistochemistry with anti-GFP and anti-NeuN antibodies, together with DAPI for nuclear staining, and they were observed using a confocal microscope (SP-8, Leica). Arrows indicate NeuN- and GFP-positive cells. Dashed lines in the low magnification images of the WT fetuses indicate the top of the cerebral wall. Inserts are enlarged images of square areas on the lower magnification micrographs. Scale bars = 250 μm (low magnification) or 50 μm (high magnification).