Table 1.
In vitro and in vivo development of embryos after lentivirus injection
| Group | APP-lentivirus injected (Fresh) | APP-lentivirus injected (Vitrified/thawed) | WT (Fresh) | ||
| 1 embryo/surrogate | 2 embryos/surrogate | 1 embryo/surrogate | 2 embryos/surrogate | 1 embryo/surrogate | |
| No. of MII oocyte used for ICSI | 518 | 134 | |||
| No. of 2 cell stage | 323 (62.4) | 104 (77.6) | |||
| No. of blastocyst (% per 2 cell) | 177 (54.8) | 65 (62.5) | |||
| No. of blastocyst vitrified | 82 | – | |||
| No. of blastocyst thawed | – | – | 54 | – | |
| No. of post-thawed blastocyst expanded | – | – | 26 (48.1) | – | |
| ETs | 29 | 46 | 8 | 10 | 22 |
| No. of surrogates | 29 | 23 | 8 | 5 | 22 |
| No. of pregnancies at 30 days after ICSI | 5 (17.2) | 7 (30.4) | 1 (12.5) | 1 (20) | 6 (27.3) |
| Twin | – | 2 | – | 1 | – |
| Spontaneous miscarriage | 2 | 6 | 0 | 1 | 3 |
| Live birth | 3 | 3 | 1 | 1 | 3 |
| GFP+offspring | 2 | 0 | 0 | 1 | – |
ICSI, intracytoplasmic sperm injection; ET, embryo transfer; GFP, green fluorescent protein.