Table 2.

List of ADAMTS‐5 substrates

Substrate	UniProt ID	Cleavage site (P1↓P1′)	References
ADAMTS‐5	Q9UNA0	Glu753↓Gly754	[44]a
Aggrecan	P16112‐1	Glu392↓Ala393	[1, 2]a
		Glu1679↓Gly1680	[48]a
		Glu1848↓Gly1849	[182]a
		Glu1953↓Ala1954	[47]a
		Glu2053↓Leu2054	[182]a
α2M	P01023	Met713↓Gly714	[183]a
Biglycan	P21810	Asn186↓Cys187	[48] [184]a
Brevican	Q96GW7‐1	Glu400↓Ser401	[152]a
CILP‐1	O75339	Glu591↓Val592	[185]a
		Val592↓Val593	[185]a
		Ser607↓Phe608	[185]a
		Trp699↓Ser700	[185]a
		Glu717↓Asn718	[185]a
		Asn718↓Gln719	[185]a
		Gln719↓Arg720	[185]a
		Asn722↓Lys723	[185]a
CILP‐2	Q8IUL8	Thr810↓Ala811	[185]a
		Ala811↓Thr812	[185]a
		Leu813↓Gly814	[185]a
		Ala830↓Thr831	[185]a
		Val832↓Gly833	[185]a
Clusterin	P10909	Asn43↓Lys44	[185]a
Cm‐tf	P02787‐1	Cys58↓Val59	[48]a
		Cys213↓Leu214	[48]a
		Ser409↓Leu410	[48]a
		Cys542↓Leu543	[48]a
Collagen type II α1	P02458	Ala902↓Gly903	[185]a
		Pro922↓Ser923	[185]a
		Ser923↓Gly924	[185]a
		Gly1053↓Val1054	[185]a
		Pro1078↓Ala1079	[185]a
Collagen type III α1	P02461	Gln374↓Gly375	[185]a
		Asn389↓Gly390	[185]a
Decorin	P07585	Lys74↓Val75	[48] [185]a [186]
		Leu85↓Leu86	[48] [185]a [186]
Fibromodulin	P13605	Tyr63↓Ala64	[186]a
Fibronectin	P02751	Phe2113↓Val2114	[48] [185]a
		Pro2135↓Ser2136	[48] [185]a
Inter‐α‐inhibitor HC2	P19823	Arg359↓Asn360	[187]a
		Thr366↓Lys367	[187]a
		Tyr490↓Asn491	[187]a
		Asn491↓Gln492	[187]a
		Leu681↓Ala682	[187]a
Prolargin	P51888	His340↓Asp341	[185]a
		His351↓Leu352	[185]a
Reelin		‐	[165]
Syndecan‐1		‐	[124]
Tenascin	P24821	Cys64↓Ser65	[185]a
		Ser70↓Ala71	[185]a
		Glu89↓His90	[185]a
Versican V1	P13611‐2	Glu441↓Ala442	[41, 97, 98]

Glutamic acid is over‐represented in P1 position.

Abbreviations: α2M, α2‐macroglobulin; CILP, cartilage intermediate layer protein; Cm‐Tf, carboxymethylated transferrin.

^aR i Cleavage sites identified by mass‐spectrometry/Edman sequencing. Note that for aggrecan, the cleavage sites reported by the original references were for the bovine homologue (UniProt ID: P13608).