Figure 4. Post hoc clustering of unassigned cells and joint classification of cell types using multiple reference datasets. (see also Fig EV4).

1. Left panel shows cell types based on the original publication by Muraro et al (2016), Data ref: Muraro et al (2016). Middle panel shows the predicted cell types from scClassify trained on the reference dataset by Xin et al (2016), Data ref: Xin et al (2016). Note that the reference dataset does not contain the cell types acinar, ductal and stellate cells. Right panel shows post hoc clustering and cell typing results for cells that remained unassigned in the scClassify prediction.
2. Joint classification on the PBMC data collection. Classifying query datasets using the joint prediction from multiple reference datasets (red circle). Classification accuracy as well as unassigned and intermediate rate of the joint prediction is compared to that obtained from using single reference datasets (other colours).