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COP1 ubiquitinates GAI and RGA. (A and B) The 6xHis-M5GAI (A) and 6xHis-RGA52 (B) ubiquitination assay using recombinant MBP-COP1, rice E2, and unmodified ubiquitin. (C) The 6xHis-M5GAI ubiquitination assay using unmodified and HA-tagged ubiquitin. Modified and unmodified 6xHis-M5GAI and 6xHis-RGA52 were detected with anti-GAI and anti-6xHis antibodies, respectively.
