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. Author manuscript; available in PMC: 2020 Jun 22.
Published in final edited form as: Acta Biomater. 2018 Apr 24;74:56–73. doi: 10.1016/j.actbio.2018.04.048

Table 1.

The effect of cartilage T-dECMs on chondrocyte/stem cell proliferation.

dECM origin Seeded cell type Treatment Analysis Results Reference
bovine AC human BMSC grown in soluble dECMs in a 2D culture system MTS assay/cell metabolism enhanced cell proliferation 22
bovine AC rabbit BMSC grown in conditioned medium from dECM extraction BrdU analysis 10 mg/mL dECM promoted cell proliferation at day 6 and 9 55
bovine meniscus human BMSC grown in conditioned medium with dECM extraction from menisci MTS assay enhanced cell proliferation 72
human AC human SDSC grown in conditioned medium from dECM extraction WST-1 (activity) and LDH (death) analyses supported cell proliferation 70
porcine AC human ADSC seeded onto the genipin-crosslinked physically prepared dECM in a culture medium without exogenous growth factors DNA content crosslinked scaffold with genipin supported cell proliferation 220
porcine AC human and porcine chondrocyte seeded onto physically prepared dECM scaffolds in a culture medium DNA content supported cell growth and proliferation 221
porcine AC human BMSC cultured on dECM scaffolds in a chondrogenic medium DNA content supported cell proliferation 76
porcine AC human IPFSC cultured on dECM scaffolds in a chondrogenic medium DNA content supported cell proliferation 74
porcine AC human IPFSC seeded onto physically prepared dECM scaffolds in a chondrogenic medium DNA content supported greater proliferation within the scaffolds fabricated using 250 mg/mL cartilage slurry concentrations 57
porcine AC (immature and mature) human IPFSC seeded onto dECMs and cultured in a chondrogenic medium DNA content supported cell proliferation 222
porcine AC rat BMSC treated with DCC in a pellet culture system DNA content supported cell proliferation (superior to DVC at day 7) 223
porcine meniscus human chondrocyte cultured on dECM scaffolds in a culture medium DNA content enhanced cell proliferation 73
porcine NP human ADSC cultured on dECM scaffolds cell number supported cell proliferation 75

Abbreviations:2D: two-dimensional; AC: articular cartilage; ADSC: adipose derived stem cell; BMSC: bone marrow stromal cell; DCC: chemically decellularized cartilage particles; dECM: decellularized extracellular matrix; DVC: physically devitalized cartilage particles; ECM: extracellular matrix; IPFSC: infrapatellar fat pad derived stem cell; LDH: lactate dehydrogenase; NP: nucleus pulposus; SDSC: synovium derived stem cell.