Table 1.
dECM origin | Seeded cell type | Treatment | Analysis | Results | Reference |
---|---|---|---|---|---|
bovine AC | human BMSC | grown in soluble dECMs in a 2D culture system | MTS assay/cell metabolism | enhanced cell proliferation | 22 |
bovine AC | rabbit BMSC | grown in conditioned medium from dECM extraction | BrdU analysis | 10 mg/mL dECM promoted cell proliferation at day 6 and 9 | 55 |
bovine meniscus | human BMSC | grown in conditioned medium with dECM extraction from menisci | MTS assay | enhanced cell proliferation | 72 |
human AC | human SDSC | grown in conditioned medium from dECM extraction | WST-1 (activity) and LDH (death) analyses | supported cell proliferation | 70 |
porcine AC | human ADSC | seeded onto the genipin-crosslinked physically prepared dECM in a culture medium without exogenous growth factors | DNA content | crosslinked scaffold with genipin supported cell proliferation | 220 |
porcine AC | human and porcine chondrocyte | seeded onto physically prepared dECM scaffolds in a culture medium | DNA content | supported cell growth and proliferation | 221 |
porcine AC | human BMSC | cultured on dECM scaffolds in a chondrogenic medium | DNA content | supported cell proliferation | 76 |
porcine AC | human IPFSC | cultured on dECM scaffolds in a chondrogenic medium | DNA content | supported cell proliferation | 74 |
porcine AC | human IPFSC | seeded onto physically prepared dECM scaffolds in a chondrogenic medium | DNA content | supported greater proliferation within the scaffolds fabricated using 250 mg/mL cartilage slurry concentrations | 57 |
porcine AC (immature and mature) | human IPFSC | seeded onto dECMs and cultured in a chondrogenic medium | DNA content | supported cell proliferation | 222 |
porcine AC | rat BMSC | treated with DCC in a pellet culture system | DNA content | supported cell proliferation (superior to DVC at day 7) | 223 |
porcine meniscus | human chondrocyte | cultured on dECM scaffolds in a culture medium | DNA content | enhanced cell proliferation | 73 |
porcine NP | human ADSC | cultured on dECM scaffolds | cell number | supported cell proliferation | 75 |
Abbreviations:2D: two-dimensional; AC: articular cartilage; ADSC: adipose derived stem cell; BMSC: bone marrow stromal cell; DCC: chemically decellularized cartilage particles; dECM: decellularized extracellular matrix; DVC: physically devitalized cartilage particles; ECM: extracellular matrix; IPFSC: infrapatellar fat pad derived stem cell; LDH: lactate dehydrogenase; NP: nucleus pulposus; SDSC: synovium derived stem cell.