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. 2020 Jun 29;92(11):2839–2846. doi: 10.1002/jmv.26171

Figure 5.

Figure 5

Specificity evaluation of one‐step RT‐PCR using coronavirus specificity panel. (A) Amplicons of E gene; lane 1: DNA ladder 100 bp; lane 2: negative control; lane 3: SARS‐CoV‐2; lane 4: SARS‐CoV; lane 5: MERS‐CoV; lane 6: HCOV‐OC43; lane 7: HCOV‐229E; lane 8: HCOV‐NL63. (B) Amplicons of RdRp gene; lane 1: DNA ladder 100 bp; lane 2: negative control; lane 3: SARS‐CoV‐2; lane 4: SARS‐CoV; lane 5: MERS‐CoV; lane 6: HCOV‐OC43; lane 7: HCOV‐229E; lane 8: HCOV‐NL63. HCOV, human coronavirus; MERS, Middle East respiratory syndrome; RT‐PCR, reverse transcription polymerase chain reaction; SARS‐CoV, severe acute respiratory syndrome coronavirus