FIG 4.

β-HPV 8E6 diminishes LATS phosphorylation during failed cytokinesis. Representative images of U2OS (A) and HFK (B) cells before and during H2CB exposure. Green and blue represent α-tubulin and DAPI, respectively. Quantification of U2OS (C) and HFK (D) cells with 2 or more nuclei as a function of time in H2CB. (E) STK4, LATS, and YAP1 expression before and after H2CB exposure measured by RT-qPCR; n = 2. (F) Representative immunoblot of pLATS and totals LATS protein levels in HFK cells before and during H2CB exposure. (G) Representative images of YAP (green)- and DAPI (blue)-stained HFK cells before and during H2CB treatment. At least 200 cells/line were imaged from three independent experiments. Figures depict mean ± standard error of the mean; n ≥ 3. †, significant difference relative to before H2CB; †, P ≤ 0.05; ††, P ≤ 0.01; †††, P ≤ 0.001 (Student's t test). In β-HPV Δ8E6, residues 132 to 136 were deleted.