FIG 4.

Association of acetylated histone-3 and viral early genes or gene promoters. Monolayers of A549 cells were uninfected or infected with HAdV2 (15 MOI) for 6 h. The samples were used to detect acetylated histone-3 to viral gene promoters by ChIP assay. Adenoviral E1, E2, E3, and E4 early genes as well as a late gene IVa2 in the anti-Ac-H3K9 and anti-Ac-H3K18 immunocomplexes were determined by qPCR measurement. Data for IVa2 were not included since they were not detected. The experiment was performed 2 times independently. The data are presented from a representative experiment.