FIG 5.

Effect of HDACi LAQ824 on histone-3 acetylation and association with viral early gene promoters. (A and B) LAQ824 treatment enhances Ac-H3K9 and Ac-H3K18. A549 cells were mock treated or treated with LAQ824 at indicated concentrations prior to HAdV2 infection (MOI, 15). The levels of hexon, Ac-H3K9, Ac-H3K18, and p-H3 were detected at 6 h p.i. by immunoblotting (A). In parallel experiments, the samples were fixed at 6 h p.i. and were used to detect histone modification by immunofluorescence (B). The experiments were performed 2 times independently; representative data are presented. (C) LAQ824 promotes Ac-H3K9 and Ac-H3K18 association with viral genes or gene promoters. A549 cells were uninfected or infected with HAdV2 (15 MOI) for 6 h in the absence or presence of 100 or 300 nM LAQ824. The samples were processed for ChIP assay by immunoprecipitating for Ac-H3K9 and Ac-H3K18. Adenoviral E1, E2, E3, and E4 early genes and late gene IVa2 in the immunocomplexes were determined by qPCR measurement. Data for IVa2 were not included since they were not detected. The experiment was performed 2 times independently. The data are presented from a representative experiment.