FIG 3.
HPV16 E7 stabilizes TP53 independently of p14ARF. Validation of p14ARF depletion in control (C) and HPV16 E7-expressing (E7) HFKs by Western blotting (A) and qRT-PCR (B). The mRNA levels of the TP53-responsive gene CDKN1A were also accessed in p14-depleted HFKs (C). The bar graph presents means and SEM (n = 3) calculated from a single representative experiment. ***, P < 0.001; ns, nonsignificant (Student's t test). Similar results were obtained in two independently derived HFK populations.