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LAMPs interact with both MuV-F and furin. HEK293 cells were transfected with pCA7-Flag-LAMP1, -LAMP2A, -LAMP3, or -EGFP and one of pCA7-HA-MuV-F, -furin, and -EGFP. pCA7-Flag-EGFP and pCA7-HA-EGFP were used as negative controls. Proteins were immunoprecipitated 24 h later with anti-Flag ab, followed by Western blotting of total lysates (Input) and immunoprecipitates (IP) with anti-Flag (upper) or anti-HA ab (lower).
