TABLE 1.

Bacterial strains and plasmids used in this study

Strains or plasmid	Relevant characteristics or descriptiona
E. coli strains
BL21	E. coli engineering bacterium used as competent cells
K-12	Wild-type strain, host bacterium of phage Bp7
K-12-R	ΔhldE mutant naturally derived from strain K-12 and resistant to phage Bp7, heptose-less LPS
K-12C-hldE	Complemented ΔhldE strain, transformed with a pMD19-T vector with cloned hldE
K-12 ΔlamB	ΔlamB mutant derived from strain K-12, CRISPR-Cas9 technology knockout of lamB gene
K-12 ΔompC	ΔompC mutant derived from strain K-12, CRISPR-Cas9 technology knockout of ompC gene
K-12 ΔwaaF	ΔwaaF mutant derived from strain K-12, CRISPR-Cas9 technology knockout of waaF gene
K-12 ΔompC ΔlamB	ΔompC ΔlamB mutant derived from strain K-12, CRISPR-Cas9 technology knockout of ompC and lamB genes
K-12-R ΔompC	ΔompC mutant derived from strain K-12-R, CRISPR-Cas9 technology knockout of ompC gene
K-12-R ΔlamB	ΔlamB mutant derived from strain K-12-R, CRISPR-Cas9 technology knockout of lamB gene
Plasmids
pKDsg-ack	sgRNA plasmid, Specr
pKDsg-LamB	Derived from sgRNA plasmid, retargeting the lamB gene, Specr
pKDsg-OmpC	Derived from sgRNA plasmid, retargeting the ompC gene, Specr
pKDsg-WaaF	Derived from sgRNA plasmid, retargeting the waaF gene, Specr
pCas9	CRISPR Cas9 plasmid, expression of Cas9 protein used as cleaving gene, Ampr
pCold-TF-LamB	Fusion cold shock expression plasmid with a TF chaperone fusion tag, expresses LamB protein, Ampr
pCold-TF-OmpC	Fusion cold shock expression plasmid with a TF chaperone fusion tag, expresses LamB protein, Ampr
pCold-TF-gp37	Fusion cold shock expression plasmid with a TF chaperone fusion tag, expresses gp37 protein, Ampr
pCold-TF-gp38	Fusion cold shock expression plasmid with a TF chaperone fusion tag, expresses gp38 protein, Ampr
pMD19-T-hldE	Cloning plasmid, expression of hldE protein, Ampr

^asgRNA, single guide RNA; Spec^r, spectinomycin resistance; Amp^r, ampicillin resistance.