FIG 5.

Viral RNA synthesis in cells with RPS6KB1 deficiency. Cells were infected at an MOI of 5, and total RNA was collected from 4 independent samples at 2 and 24 hpi. (A) Genome replication of MuV (vRNA) in WT and RPS6KB1-KO cells. Genome-specific primers were used to detect genomic RNA. (B) MuV mRNA. Oligo(dT) primers were used to generate the mRNAs. (C) Viral mRNA relative to genomic RNA. The ratio of vmRNA to genomic vRNA was calculated and then normalized to the WT HapI control. A MuV F-specific probe was used for real-time PCR after cDNA synthesis. The values were calculated after normalization to genomic-RNA levels at 2 hpi. P values were calculated using multiple T tests. *, P < 0.5; **, P < 0.01. The error bars represent SEM of data from 4 replicates of 3 individual experiments.