FIG 6.

MuV P and NP phosphorylation in cells with RPS6KB1 deficiency. (A) Representative radio blot. HeLa cells were transfected with siRNA, infected with MuV at an MOI of 0.1, and then labeled with ³⁵S or ³³P for 6 to 8 h. Cell lysates were immunoprecipitated with monoclonal anti-MuV P or anti-MuV NP antibody and resolved on an SDS-PAGE gel. (B) Total P protein quantification. Shown is ³⁵S-labeled P protein from infected HeLa cells. Values were standardized to that of the nontarget control. (C) Relative phosphorylation levels of P in infected cells. The relative level was calculated as a ratio of phosphorylated protein (³³P) to total protein (³⁵S) and standardized to the nontarget control. P values were calculated using Student's t test. *, P < 0.5. The error bars represent SEM of data from 3 individual experiments.