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. 2020 Jun 1;94(12):e00408-20. doi: 10.1128/JVI.00408-20

FIG 2.

FIG 2

Reactivities of MAb 5A5 IgG, mIgA, and pSIgA to HA antigens from various subtypes. Reactivities of MAb 5A5 IgG, mIgA, and pSIgA to recombinant HAs were measured in ELISA. MAb 5A5 was diluted at 0.1 μg/ml before use. HAs of the following IAV strains were used: A/swine/Hokkaido/2/1981 (H1N1), A/Puerto Rico/8/1934 (H1N1), A/Kadoma/4/2006 (H1N1), A/Narita/1/2009 (H1N1), A/Adachi/2/1957 (H2N2), A/Aichi/2/1968 (H3N2), A/duck/Hokkaido/5/1977 (H3N2), A/duck/Czechoslovakia/1956 (H4N6), A/Hong Kong/483/1997 (H5N1), A/duck/Hong Kong/820/1980 (H5N3), A/shearwater/South Australia/1/1972 (H6N5), A/duck/Hokkaido/301/1978 (H7N2), A/seal/Massachusetts/1/1980 (H7N7), A/turkey/Ontario/6118/1968 (H8N4), A/Hong Kong/1073/1999 (H9N2), A/chicken/Germany/N/1949 (H10N7), A/duck/England/1/1956 (H11N6), A/duck/Alberta/60/1976 (H12N5), A/gull/Maryland/704/1977 (H13N6), A/mallard/Astrakhan/263/1982 (H14N5), A/duck/Australia/341/1983 (H15N8), A/black-headed gull/Sweden/5/1999 (H16N3), and A/little yellow-shouldered bat/Guatemala/060/2010 (H17N10). An influenza B virus strain, B/Lee/1940, was used as a negative control. Columns and error bars indicate the means and standard deviations of triplicate wells, respectively.