FIG 6.

Detection of the viral protein in supernatants and lysates of IAV-infected cells. MDCK cells were infected with IAVs at an MOI 2.0 and incubated with or without 5A5 and B12 MAbs (10 μg/ml). The M1 protein in supernatants (A) and cell lysates (B) was detected in Western blotting and beta-actin was also stained for cell lysate samples. The band intensities relative to each control sample (i.e., cells incubated without MAbs) are shown. Each experiment was performed three times, and averages and standard deviations are shown. Asterisks indicate significant differences (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001) determined using one-way ANOVA, followed by Tukey’s multiple-comparison tests.