FIG 2.

Functional analysis of P_ΔOD in vitro. Reactions were performed in the absence (Ø) or presence of equimolar amounts of P_WT or P_ΔOD using purified L and either a synthetic, naked 19-nt RNA template corresponding to the 3′ leader sequence of the VSV genome (A) or a purified, encapsidated N-RNA template (B). Radioactive products were analyzed by gel electrophoresis on a 20% acrylamide gel (A) or a 1.75% acid-agarose gel containing 6 M urea (B). (A) n = 1 replicate; (B) representative experiment (n = 3 replicates).