FIG 4.

VFs decrease in number and increase in size over time. DF-1 cells were either transfected with GFP1-10 and infected with the PBG98-VP1-GFP11 virus (A) or infected with the wt PBG98 virus (B) an MOI of 1 and fixed at 10, 18, and 24 hpi, stained with DAPI, and imaged. The wt PBG98-infected cells were also stained with mouse anti-VP3 and goat anti-mouse-568 for visualization of VFs. Scale bars, 20 μm. The number of VFs per cell was determined for 30 infected cells at each time point and plotted for cells infected with the PBG98-VP1-GFP11 virus (C) and the wt PBG98 virus (E). The average area of the VFs in an infected cell was determined using the surface tool in Imaris 9 software (bitplane) and plotted for 30 infected cells at each time point for cells infected with the PBG98-VP1-GFP11 virus (D) and the wtPBG98 virus (F). The line represents the mean, and the error bars represent the SD of the mean. The numbers and sizes of foci were compared using Kruskal-Wallis one-way ANOVA. ***, P < 0.001; ****, P < 0.0001.