FIG 7.

VF coalescence is dependent on an intact microtubule network and actin cytoskeleton. DF-1 cells were transfected with a plasmid expressing GFP1-10 and infected 24 h posttransfection with the PBG98-VP1-GFP11 virus at an MOI of 1. Cells were treated with either nocodazole (A) or cytochalasin-D (B) from 2 hpi. One live infected cell was imaged every 4 min from 20 to 22 hpi. Images of the boxed region are shown at the indicated time points postinfection. (A) In an infected cell treated with nocodazole, VFs were witnessed transiently interacting (white arrows). (B) In an infected cell treated with cytochalasin-D, four VFs were observed (white, blue, red, and purple arrows) that did not coalescence or interact with each other over the 2-h period. Scale bars, 20 μm.