FIG 3.

ANDV-NSs is detected in the lungs of experimentally inoculated golden Syrian hamsters. Male golden Syrian hamsters were inoculated with a mock control or with the Andes/ARG strain of ANDV as described in the Materials and Methods. Shown are lung samples collected from representative animals in each group, necropsied at day 8 postinoculation with a mock sample or with the virus and tested for the presence of the N and NSs viral proteins by immunohistochemistry. (A) Lung tissue sample obtained from mock-inoculated animals was processed for the ANDV-N (far left) or the ANDV-NSs (middle) proteins using an anti-N (94) or an anti-NSs (9) antibody, respectively. Lungs obtained from an animal infected with the Andes/ARG strain of ANDV were stained using the same procedure but omitting the addition of the primary anti-ANDV protein antibody and using only the secondary antibody (far right). (B) Lung tissue samples obtained from three independent animals infected with the Andes/ARG strain of ANDV were tested for the presence of the N and NSs viral protein by immunohistochemistry using an anti-N (94) or an anti-NSs (9) antibody. The cellular nuclei of the lung tissues were stained with hematoxylin-eosin. Bars, 50 μm.