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The HA-NSs protein expression does not impact on the levels of endogenous or recombinant MAVS. HEK 293T cells were transfected with different amounts of HA-NSs expression plasmid (A and B) or together with a constant amount of FLAG-MAVS (B). At 24 h p.t., cells were lysed and Western blotting was performed using mouse anti-MAVS, mouse anti-HA, and mouse anti-GAPDH primary antibodies (A) or mouse anti-FLAG, mouse anti-HA and mouse anti-GAPDH primary antibodies (B).
