FIG 1.

Frequency of B*52:01-restricted mutations and the association between CD4 count and HLA-B alleles (n = 138). (A) Association between HLA-B alleles and CD4 counts. Vertical bar represents the median CD4 counts, boxes indicate the IQR, and brackets are the maximum and minimum values. The median CD4 count is marked on the top of each bar. A Kruskal-Wallis test was applied (P = 0.004). Here, the individual P value was corrected by Bonferroni’s correction (P value threshold of 0.0038). The asterisk represents a statistically significant difference after Bonferroni correction for multiple tests between the absolute CD4 count in individuals expressing a particular HLA allele and in the remaining cohort. (B) Comparison of the percentages of the wild type (WT) and V280X variants (where X is T, S, or A) at Gag-280 in HLA-B*52:01-positive (B52+ve) and -negative (B52−ve) individuals. P < 0.0001 (Fisher’s exact test); q = 0.006. (C) CD4 counts of each V280X mutant in HLA- B*52:01-positive and -negative individuals. Bar represents the median of CD4 counts. A Mann-Whitney U test and Steel-Dwass test were performed for post hoc analysis (P < 0.05 of V280A compared to results with the wild type). **, P < 0.01; ***, P < 0.001.