TABLE 5.
HLA-B*52:01 custom-designed mutagenesis forward and reverse primers
| HXB2 position in Gag | Clade | Mutant | Primer direction | Primer sequence (5′→3′)a |
|---|---|---|---|---|
| 147 | C | I→L | Forward | CAA ATG GTA CAC CAA GCC TTA TCA CCT AGA ACT TTG AA |
| C | Reverse | TT CAA AGT TCT AGG TGA TAA GGC TTG GTG TAC CAT TTG C | ||
| 256 | C | I→V | Forward | G ATG ACT AGT AAC CCA CCT GTC CCA GTG GGA G |
| C | Reverse | C TCC CAC TGG GAC AGG TGG GTT ACT AGT CAT C | ||
| 260 | C | D→E | Forward | CA CCT ATC CCA GTG GGA GAG ATC TAT AAA AGA TGG ATA AT |
| C | Reverse | AT TAT CCA TCT TTT ATA GAT CTC TCC CAC TGG GAT AGG TG | ||
| 280 | B | T→V | Forward | AAA ATA GTA AGA ATG TAT AGC CCT GTC AGC ATT CTG GAC ATA AGA CAA GG |
| B | Reverse | CC TTG TCT TAT GTC CAG AAT GCT GAC AGG GCT ATA CAT TCT TAC TAT TTT | ||
| B | T→S | Forward | A GTA AGA ATG TAT AGC CCT AGC AGC ATT CTG GAC ATA AG | |
| B | Reverse | CT TAT GTC CAG AAT GCT GCT AGG GCT ATA CAT TCT TAC T | ||
| B | T→A | Forward | ATA GTA AGA ATG TAT AGC CCT GCC AGC ATT CTG GAC ATA AG | |
| B | Reverse | CT TAT GTC CAG AAT GCT GGC AGG GCT ATA CAT TCT TAC TAT | ||
| C | V→T | Forward | T AAA ATA GTA AGA ATG TAT AGC CCT ACC AGC ATC TTG GAC ATA AAA CAA GGG | |
| C | Reverse | CCC TTG TTT TAT GTC CAA GAT GCT GGT AGG GCT ATA CAT TCT TAC TAT TTT A | ||
| C | V→S | Forward | AAA ATA GTA AGA ATG TAT AGC CCT AGC AGC ATC TTG GAC ATA AAA CAA GGG | |
| C | Reverse | CCC TTG TTT TAT GTC CAA GAT GCT GCT AGG GCT ATA CAT TCT TAC TAT TTT A | ||
| C | V→A | Forward | A GTA AGA ATG TAT AGC CCT GCC AGC ATC TTG GAC ATA AAA C | |
| C | Reverse | G TTT TAT GTC CAA GAT GCT GGC AGG GCT ATA CAT TCT TAC T | ||
| 286 | B | R→K | Forward | C AGC ATT CTG GAC ATA AAA CAA GGA CCA AAG GAA CCC |
| B | Reverse | GGG TTC CTT TGG TCC TTG TTT TAT GTC CAG AAT GCT G | ||
| C | K→R | Forward | C AGC ATC TTG GAC ATA AGA CAA GGG CCA AAG GAA CC | |
| C | Reverse | GG TTC CTT TGG CCC TTG TCT TAT GTC CAA GAT GCT G |
a
Mutant amino acids are shown in boldface.