TABLE 1.

Genetic variations observed within the RBS of H5N1 during human infection

Domain	H3 no.a	H5 no.a	Ind/05b	No. of samplesc	Residue(s) (no. of samples [%])d
Conserved interface residue	95 (98)	91	Y	12	Y: 12 (100)
130-loop	132	128	S	12	S: 12 (100)
	133	129	S	12	S: 12 (92–100); P: 1 (8)
	134	130	G
	135	131	V
	136	132	S
Conserved interface residue	153	149	W	60	W: 60 (97–100)
Antigenic site	155	151	I	60	I: 60 (98–100) M: 1 (1), N: 1 (2)
Conserved interface residue	183	179	H	59	H: 59 (98–100); R: 1 (2)
190-helix	186	182	N	59	N: 59 (95–100); S: 3 (3–5); H: 1 (3)
	187	183	D	59	N: 40 (25–100); D: 19 (75–100); T: 1 (100)
	188	184	A	59	E: 38 (3–100); A: 18 (75–100); G: 3 (95–97); K: 3 (2–100); V: 2 (100); T: 1 (6)
	189	185	A	59	A: 58 (98–100); E: 1 (99); K: 1 (1); T: 1 (2)
	190	186	E	59	E: 59 (92–100); G: 5 (1–4); A: 1 (3); K: 1 (3)
	191	187	Q	59	Q: 59 (99–100)
	192	188	T	59	T: 59 (98–100); A: 2 (2–2)
	193	189	R	59	M: 37 (26–100); R: 17 (74–100); K: 6 (100)
	194	190	L	59	L: 59 (99–100)
Conserved interface residue	195	191	Y	59	Y: 59 (98.33–100)
220-loop	224	220	N	59	N: 59 (94–100); D: 1 (4); H: 1 (1); K: 1 (6); S: 1 (3)
	225	221	G	59	G: 59 (82–100); E: 2 (2–2.3); R: 1 (18)
	226	222	Q	59	Q: 59 (80–100); R: 4 (2–20); K: 1 (5)
	227	223	S	59	S: 59 (97–100); R: 1 (1)
	228	224	G	59	G: 59 (97–100); R: 1 (3)

^aPosition according to H3 and H5 numbering after removal of the signal peptide.

^bAmino acid present in the sequence of reference strain A/Indonesia/5/2005 used in this work.

^cNumber of samples with sequencing data available at the position mentioned.

^dResidues observed, number of samples in which the amino acid is observed, and proportion or proportion range within the sample(s).