FIG 3.

The amount of infectious RSV is decreased in infected ciliated epithelial cells exposed to neutrophils. (A) The amount of infectious virus present in whole wells following neutrophil coculture as measured by plaque forming units per milliliter (PFU/ml). (B) Whole-well scan of a representative membrane insert infected with RSV for 24 h or 72 h cells before (left) and 1 h after (right) neutrophil exposure. Each white spot indicates an RSV-infected epithelial cell. (C) Mean fluorescence intensity of GFP-RSV-infected AECs for 24 h or 72 h cells after 1 h without (clear bars) and with (spotted bars) neutrophils. Bars represent the mean ± SEM (n = 6 epithelial donors with heterologous neutrophils. P values show a significant difference between the matched pre- and post-neutrophil exposure time points.