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Properties of stabilized BG505 DS-SOSIP.664 Env trimers. (A) Size exclusion chromatography (SEC) profiles of stabilized DS-SOSIP.664 variants before V3-negative selection. (B) SDS-PAGE of stabilized DS-SOSIP.664 variants after V3-negative selection, with and without the presence of dithiothreitol (DTT). The unit of measure of the SDS-PAGE marker is kDa. (C) Expression yield of stabilized DS-SOSIP.664 variants after SEC alone or after SEC with V3-negative selection.
