FIG 4.

Defective viral RNAs increase surface HLA presentation in a MAVS-dependent manner. (A) A549 cells or A549-MAVS-KO cells were transfected with IAV minireplicon expressing mvRNA from genome segment 5 or empty pUC19 control for 24 h prior to harvest of protein lysates and immunoblotting with antibodies for the indicated target proteins. (B) A549 cells or A549 MAVS-KO cells were transfected with IAV minireplicon expressing defective vRNAs from genome segment 5 and analyzed by flow cytometry at 48 h posttransfection via surface immunostaining with a pan-anti-HLA-A/B/C antibody (n = 3). Histograms from a representative experiment are shown on the left; the vertical lines indicate the expression level of the target in uninfected cells. On the right, relative MFI values from at least 3 independent experiments are shown. *, P < 0.05.