TABLE 1.
Primer sequences for RT-qPCR analysis
| RT-qPCR target | Primer sequences (5′–3′) | Source |
|---|---|---|
| HLA-A | F-CGACGCCGCGAGCCAGA, R-GCGATGTAATCCTTGCCGTCGTAG | 63 |
| HLA-B | F-GACGGCAAGGATTACATCGCCCTGAA, R-CACGGGCCGCCTCCCACT | 63 |
| HLA-C | F-GGAGACACAGAAGTACAAGCG, R-CGTCGTAGGCGTACTGGTCATA | 63 |
| HLA-E | F-CCTACGACGGCAAGGA, R-CCCTTCTCCAGGTATTTGTG | 63 |
| MIC-A | F-ACTTGACAGGGAACGGAAAGGA, R-CCATCGTAGTAGAAATGCTGGGA | 63 |
| MIC-B | F-ATCTGTGCAGTCAGGGTTTCTC, R-TGAGGTCTTGCCCATTCTCTGT | 63 |
| PVR | F-GCTCTGCTGTTTGTTCTGCTTTCC, R-TTTCTGCTGCTGGATGCGGTTT | 63 |
| NECTIN1 | F-ACTACCACATGGACCGCTTC, R-GTTGATGGGTCCCTTGAAGA | Designed in-house |
| NECTIN2 | F-TGGACTGGGAAGCCAAAGAGA, R-TACAGAGAGGGTCACAGGTATCAGG | 63 |
| NECTIN3 | F-GTTACATTCCCGCTTGGAAA, R-CCCAGTCAATATGTGCAACG | Designed in-house |
| CADM1 | F-GGTGGAAGAGTGGTCAGACA, R-CTTCCCGATGGCTTCACATG | Designed in-house |
| CDH1 | F-AGGAATCCAAAGCCTCAGGT, R-ACCCACCTCTAAGGCCATCT | Designed in-house |
| CDH2 | F-GAGGCAGAGACTTGCGAAAC, R-CCATTAAGCCGAGTGATGGT | Designed in-house |
| PCNA | F-CGGATACCTTGGCGCTAGTA, R-CACTCCGTCTTTTGCACAGG | Designed in-house |
| CD27L | F-TGGTACACATCCAGGTGACG, R-AGGCAATGGTACAACCTTGG | Designed in-house |
| IFN-β | F-GTCTCCTCCA AATTGCTCTC, R-ACAGGAGCTTCTGACACTGA | 64 |
| IFN-λ1 (IL-29) | F-GAAGCAGTTGCGATTTAGCC, R-GAAGCTCGCTAGCTCCTGTG | 65 |
| IFN-λ2 (IL-28A) | F-GCCAAAGATGCCTTAGAAGAG, R-CAGAACCTTCAGCGTCAGG | 66 |
| 5S rRNA | F-GCCCGATCTCGTCTGATCT, R-AGCCTACAGCACCCGGTAT | Designed in-house |
| GAPDH | F-ACGAATTTGGCTACAGCAACAGGG, R-TCTACATGGCAACTGTGAGGAGG | 37 |