FIG 1.

Identification of gQ1 binding by immunoprecipitation and mass spectrometry analysis. (A) 293T cells were transfected with a plasmid for expression of gQ1. The cells were lysed and immunoprecipitated with an anti-gQ1 antibody or an isotype control antibody. The immunoprecipitates were separated in a denaturing gel and silver stained. (B) gQ1-expressing or its control cells were used for the immunoprecipitation assay using anti-gQ1 and an isotype control antibody followed by immunoblot analysis.