FIG 5.

MLAV VP40 is capable of forming virus-like particles from both human and bat cells. To compare the budding of EBOV, MARV, and MLAV VP40 proteins from different cell lines, VLP assays were performed in a HEK293T cells (A) and RO6E cells (B). Ten percent of each VLP preparation was subjected to treatment with trypsin (Tryp) or trypsin and Triton X-100 (Tryp + Triton) to determine whether VP40 was contained within a membrane. The presence of VP40 in nontreated (N.T.) and treated VLPs and WCLs was assessed by Western blot analysis with anti-FLAG antibody. Anti-β-tubulin served as a loading control for the WCL.