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Characterization of A549 and derived cell lines following IFN treatment or infection with a DVG-rich preparation of PIV5-VΔC (vM2). A549, A549/V, A549/NPro, A549/V/Npro, and A549/shIFIT1 cells were treated with IFN-β (1,000 IU/ml), infected with PIV5-VΔC vM2, or left untreated (UI). Cell lysates were prepared after 16 h and subjected to immunoblotting for IRF3, IFIT1, STAT1, the P protein of PIV5, and actin. The first 9 lanes of this figure are reprinted from reference 18.
