FIG 3.

G3BP1 interacts with PKR and Rig-I, but not OAS and RNase L, in avSGs. (A) Schematic of avSG purification and analysis of fractions in immunoblots using the indicated antibodies. (B to D) HT1080 WT, G3BP1 KO, or RNase L KO cells were mock treated or treated with 10 μM 2-5A (B), treated with RNase L-cleaved small RNAs or control small RNAs (2 μg/ml) (C), or treated with 1 mM H₂O₂ or mock treated (D), and avSGs were isolated as described in Materials and Methods. The avSG core proteins were immunoprecipitated with G3BP1 antibody, and the immune complex was analyzed for the presence of PKR, Rig-I, OAS, RNase L, and MAVS (IPS-1) by immunoblot analysis. Pellet 1 and SG core (Sup 4) fractions were probed for expression of G3BP1 and PKR, Rig-I, OAS, RNase L, and MAVS (IPS-1). Nonspecific lanes were cropped to generate the images, and the boundaries are indicated. The data are representative of results from two experiments.