FIG 6.

Induction of proinflammatory cytokines by RNase L is independent of avSG assembly. (A and B) WT and G3BP1 KO cells were transfected with CCL5-luc, IL-8-luc, or IP-10-luc and β-galactosidase plasmids and 24 h later treated with 2-5A (10 μM) (A) or RNase L-cleaved small RNAs or control small RNAs (B), and luciferase activity was normalized to β-galactosidase levels. (C and D) WT and G3BP1 KO cells were transfected with 2-5A (10 μM) (C) or RNase L-cleaved small RNAs or control small RNAs (D), and CCL5, IL-8, IP-10, and CXCL1 mRNA levels were measured by qRT-PCR and normalized to GAPDH mRNA levels. (E) WT and G3BP1 KO cells were transfected with CCL5-luc, IL-8-luc, or IP-10-luc and β-galactosidase plasmids and 24 h later treated with 100 ng/ml of TNF-α, and luciferase activity was normalized to β-galactosidase levels. P values for induction by RNase L-cleaved small RNAs compared to control small RNAs are shown. The data represent means + SE for three independent experiments. n.s, not significant.