. 2020 Jun 16;94(13):e00205-20. doi: 10.1128/JVI.00205-20

TABLE 2.

Schematic presentation of the coding regions of RNase L, G3BP1, RIG-I, PKR, and RIG-I/PKR dKO that are targeted by CRISPR-Cas9

Gene	Sequence type^a	Sequence^b
RNase L	REF	`ATGGGGCCACGCCT``TTTATCCTCGCAGCGATTGCGGGG` `AGCGTGAAGCTGCTGAAACTTTTCCT`
RNase L	MUT	`ATGGGGCCACGCCTTTTATCCTC` `-----------------------` `GCTGCTGAAACTTTTCCT`
G3BP1	REF	`GGTTGAATTGACCAAAGCAATGGTGATGGAGAAGCCTAGTCCC``CTGCTGGTCGGGCGGGAAT` `TTGTGA`
G3BP1	MUT	`GGTTGAATT` `-------------------------------------------------` `GAATTTGTGA`
RIG-I	REF	`TTCCAGG`*`ATTATATCCGGAAG-` `ACCC`**`TGG` `ACCCTACCTACA`
RIG-I	MUT	`TTCCAGGATTATATCCGGAAGAACCCTGGACCCTACCTACA`
PKR	REF	`CTAATTCA`*`GGACCTCCACATGA-` `TAGG`**`AGG` `TAGGTTGC`
PKR	MUT	`CTAATTCAGGACCTCCACATGATTAGGAGGTAGGTTGC`
RIG-I/PKR dKO^c	REF	`TTCCAGG`*`ATTATATCCGGAAG-` `ACCC`**`TGG` `ACCCTACCTACA`
	MUT	`TTCCAGGATTATATCCGGAAGAACCCTGGACCCTACCTACA`
	REF	`CTAATTCA`*`GGACCTCCACATGA-` `TAGG`**`AGG` `TAGGTTGC`
	MUT	`CTAATTCAGGACCTCCACATGATTAGGAGGTAGGTTGC`

REF, reference sequence; MUT, mutated sequence.

The reference sequence and the mutated sequence for each gene are shown, as confirmed by sequencing. The boldface letters denote the protospacer (sgRNA-binding site); the underlined letters indicate the PAM (protospacer-adjacent motif).

The top two sequences are RIG-I; the bottom two sequences are PKR.