Figure 1.

MAP3K19 activates MAPK family members. A–D, HEK293T (A), LK2 (B), MCF7 (C), or HCT-116 (D) cells were transiently transfected with empty vector (EV), WT, or KD-K1089M MAP3K19. Phosphorylation status of MAPK family members was assessed by Western blotting. Band density was quantified by ImageJ software. The data are shown as mean phospho:total protein density normalized to EV control ± S.D. *, p < 0.05; **, p < 0.01. E and F, HEK293T (E) or LK2 (F) cells were transfected with EV, WT, or KD MAP3K19, and lysates were treated with 0 or 1 μl of λ-PP. Western blotting was performed.