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METTL15 localizes in the mitochondria. A, phylogenetic analysis demonstrating that METTL15 is likely an evolutionarily conserved m4C methyltransferase from fruit fly to human but absent in worm and yeast. B, RIP-qPCR analysis of the interactions between METTL15-HA and the indicated RNA species. C, fluorescence confocal analysis of the subcellular location of WT, mitochondria-targeting signal deletion METTL15 (red), and HSP60 (green) was used as a marker for mitochondria.
