Skip to main content
. 2020 May 6;295(25):8575–8588. doi: 10.1074/jbc.RA119.011979

Figure 3.

Figure 3.

The C-terminal domain of tamalin alone is not sufficient for the ligand-mediated endocytosis of mGluR1. A and B, Acute knockdown of endogenous tamalin and replacement of endogenous tamalin with C-Tam, as shown by Western blotting (A) and quantitation of the Western blots (B). C and D, Representative images (C) and quantitation (D) showing no effect on the surface expression of Myc-mGluR1 in shTam and shTam:C-Tam-expressing neurons (n values: control, 44; shTam, 43; shTam:C-Tam, 44). E and F, Representative images (E) and quantitation (F) suggested that knockdown of the endogenous tamalin inhibited the R,S-DHPG-mediated endocytosis of Myc-mGluR1, and replacement of the endogenous tamalin with C-Tam failed to rescue the normal internalization of the receptor (n values: control, 44; control + DHPG, 44; shTam + DHPG, 42; shTam:C-Tam + DHPG, 42). The results are presented as means ± S.E. from three independent experiments. Scale bar, 10 μm. ***, p < 0.001; *, p < 0.05; n.s, p > 0.05.