Figure 3.

E2/ERα does not alter uterine loops. A, Juicer output showing the number of loops called from each sample in parentheses and differential loops called between the indicated pairs. Minimal differences are seen between replicates (V-1, V-2, V-3; P4-1, P4–2) as well as between V and hormone treatment (E2 or P4) or between mice lacking ERα (ERαKO) and WT litter mates (V-2). B, an “atlas” of uterine loops was built from the six combined WT sample Hi-C data sets. This collapsed set of loops was then filtered to retain only those with SMC1a ChIP-Seq peaks at both loop ends using either V- or E2-treated SMC1a ChIP-Seq data. The heatmap shows SMC1a signal centered on V (left) or E2 (right) SMC1a peaks that are (top) or are not (bottom) at loop ends of the selected loops. Each panel shows ChIP-Seq signal at ±1 kb relative to the SMC1a peak midpoint, with signal depth-normalized to 20 million uniquely mapped nonduplicate reads/sample