Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May 8;295(25):8602–8612. doi: 10.1074/jbc.AC120.013646

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Singh et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

Figure 3. — Deletion of IGF2BP1 from IECs increases the intestinal permeability in mice. A, LPS-specific IgG levels in the serum of Villin-CreER^T2-Igf2bp1^fl/fl (n = 5) and Igf2bp1^fl/fl (n = 5) mice. Rag1 knockout mice (n = 5) were taken as a negative experimental control. The data are means ± S.D. (error bars). B, analysis of intestinal epithelial permeability of mice by measuring FITC-dextran levels in the serum of mice Villin-CreER^T2-Igf2bp1^fl/fl (n = 7) and Igf2bp1^fl/fl (n = 7). The data are means ± S.D. C, immunoblot of tight-junction proteins in mouse intestinal epithelium (representative picture of three independent experiments). Shown is whole-cell extract from IECs of Igf2bp1^fl/fl (n = 3) and Villin-CreER^T2-Igf2bp1^fl/fl (n = 3) mice analyzed for occludin, CLAUDIN-1, CLAUDIN-2, ZO-1, and ZO-3 protein expression. β-Actin was evaluated as loading control. D, colon sections with occludin staining (green) showing the level of occludin expression in Igf2bp1^fl/fl (n = 5) and Villin-CreER^T2-Igf2bp1^fl/fl (n = 3) mice. Scale bar, 10 μm. Images are quantified for occludin by ImageJ software, and data are means ± S.D. **, p < 0.01; ***, p < 0.001. N.D., not detected; RFU, relative fluorescence units.