KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal anti-GFP	ThermoFisher Scientific	A21311; RRID: AB_221477
Alexa Fluor 488 goat anti-rabbit	ThermoFisher Scientific	A11008; RRID: AB_143165
Alexa Fluor 633 goat anti-mouse	ThermoFisher Scientific	A21050; RRID: AB_2535718
Alexa Fluor 568 phalloidin	ThermoFisher Scientific	A12380; RRID: AB_2759224
Mouse mAb anti-Bruchpilot (nc82)	Developmental Studies Hybridoma Bank	nc82; RRID: AB_2314866
Chemicals, Peptides, and Recombinant Proteins
All-trans-retinal	Sigma-Aldrich	R2500
Deposited Data
Raw and analyzed data	This paper	http://dx.doi.org/10.17632/kp9hbmxn47.1
Experimental Models: Organisms/Strains
D. melanogaster: DB331-GAL4	FlyBase	FBti0115113
D. melanogaster: GMR12C07-GAL4	Bloomington Drosophila Stock Center	RRID: BDSC_48496
D. melanogaster: GMR22H05-GAL4	Bloomington Drosophila Stock Center	RRID: BDSC_49002
D. melanogaster: ; P{y+t7.7 w+mC=20XUAS-IVS-GCaMP6f}attP40	Bloomington Drosophila Stock Center	RRID:BDSC_42747
D. melanogaster: ; P{y+t7.7 w+mC=10XUAS-IVS-myr::tdTomato}attP2	Bloomington Drosophila Stock Center	RRID:BDSC_32221
D. melanogaster: ;UAS-GCaMP6f; UAS-tdTomato	Constructed from above two lines	N/A
D. melanogaster: SS36076-SplitGAL4	Gift from G. Card, E. Ehrhardt, and W. Korff	N/A
D. melanogaster: SS41075-SplitGAL4	Gift from G. Card, E. Ehrhardt, and W. Korff	N/A
D. melanogaster: SS43980-SplitGAL4	Gift from G. Card, E. Ehrhardt, and W. Korff	N/A
D. melanogaster: tp1-SG	Gift from A. von Philipsborn [32]	N/A
D. melanogaster: Empty-SplitGAL4(; P{w[+mC]=BP-p65ADzpUw}attP40 and P{w[+mC]=BP-ZpGal4DBDUw}attP2)	Gift from J. Simpson [52]	N/A
D. melanogaster: ; P{y+t7.7 w+mC=20XUAS-IVS-CsChrimson.mVenus}attP2	Bloomington Drosophila Stock Center	RRID:BDSC_55136
Software and Algorithms
Python 2.7	https://www.python.org/	RRID: SCR_008394
Matplotlib	https://matplotlib.org/	RRID: SCR_008624
FIJI	NIH (https://fiji.sc/)	RRID:SCR_002285