Figure 6.
Quantification of cfDNA copy number by ddPCR and comparison with qPCR. A, B, The overall trend (A) and liner correlation (B) between qPCR and ddPCR. All detection values of each method represent that all seven patients' extracted CSF and serum were amplified by all six primer pairs (n = 84), ****P ˂ .0001. C, D, Extracted CSF was amplified with primer pairs of ND1 (C) and GAPDH (D). E, F, Extracted serum was amplified with primer pairs of ND1 (E) and GAPDH (F). X‐axis represents primer pairs, and Y‐axis represents log10 of copies/µL in initial raw samples. *P ˂ .05, **P ˂ .01, ***P ˂ .001. Data are presented as mean ± SD, n = 7