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. Author manuscript; available in PMC: 2020 Jul 17.
Published in final edited form as: J Am Chem Soc. 2019 Jul 8;141(28):11059–11070. doi: 10.1021/jacs.9b02679

Figure 7.

Figure 7.

Binding selectivities of MycG4-interactive indenoisoquinolines. Competition fluorescence displacement experiments with increasing concentrations of unlabeled G4s and dsDNA added to 3′-TAMRA-labeled MycPu22 (20 nM) mixed with 1 equivalent of compound 13 (A), 5 (B), 6 (C), 9 (D), and 12 (E). The normalized TAMRA fluorescence intensities at 580 nm were plotted as a function of molar ratio of added G4 DNA (in 3 G-tetrads) or calf thymus dsDNA (in 11 bp) to labeled MycPu22 DNA. The fluorescence intensity of free 3′-TAMRA labeled MycPu22 was defined as 100%, and 1:1 mixture of 3′-TAMRA labeled MycPu22 and indenoisoquinoline was defined as 0%. Conditions: 20 °C, pH 7, 100 mM K+.