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. 2020 Jun 19;8:e9203. doi: 10.7717/peerj.9203

Figure 3. DAXX mediated high Pi-induced endothelial cell apoptosis.

Figure 3

(A, C and E) qPCR and Western blot analysis for DAXX expression level in HUVECs cells after overexpression with DAXX. (B, D and F) qPCR and western blot analysis for DAXX expression level in HUVECs cells after DAXX inhibition. (G–I) Cell apoptosis was assessed using flow cytometric analysis in normal Pi environment, and high Pi in the presence or absence of siDAXX. (J) The apoptosis rate was quantified by FACS software, and data are represented as the mean ± SEM of at least three independent experiments. *p < 0.05. **p < 0.01.